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  • DNA SYNTHESIS

DNA Synthesis Services (OGAB™)

Our OGAB™ method is the only technology in the world that uses Bacillus subtilis for DNA synthesis. By using OGAB™, we can synthesize DNA for difficult sequences with characteristics such as long chains over 100 kb, extremely high GC/AT content, or repeat sequences. We can also handle sequences that cannot be cloned in E. coli. In addition, we design and construct large-scale and diverse DNA libraries to meet our clients' needs using Combinatorial-OGAB™ (Combi-OGAB™) method. All the synthesis work is carried out in-house in Japan (Kobe), ensuring thorough information management.

Plasmid guaranteed yield: 1 μg (up to mg scale is available at an additional cost)
Insertion into a specified vector (whole synthesis) is also available at an additional cost.

In addition to the above, if the synthesis is extremely difficult or if there are additional requests for purification, test, or anything else, additional cost will be incurred after further consultation.
* Synthesized DNA can only be used for R&D purposes.

Length Price(¥/bp) Additional Cost Delivery
(Working days)		 Advantages
-2 kb 180,000 Fixed TBD 10-15 Ultracentrifugal Plasmid Purification+Production in Japan
2-5 kb 90 TBD 10-15 Ultracentrifugal Plasmid Purification + Production in Japan
5-10 kb 110 TBD 15-20 Ultracentrifugal Plasmid Purification + Production in Japan
10-20 kb 130 TBD 20-30 Ultracentrifugal Plasmid Purification + Production in Japan + Delivery Time
20-50 kb 150 TBD 30-40 Ultracentrifugal Plasmid Purification + Production in Japan + Delivery Time
50-100 kb 200 TBD 40-50 Ultracentrifugal Plasmid Purification + Production in Japan + Delivery Time
100 kb- 250- TBD 50-65 Ultracentrifugal Plasmid Purification + Production in Japan + Delivery Time

Ultracentrifugal Plasmid Purification

Ultracentrifugal plasmid purification is carried out using CsCl/EtBr density gradient ultracentrifugation method.
• Transfection into animal cells   • Template plasmids for mRNA IVT
• Raw plasmids for kit production
We guarantee 100% sequence accuracy for the synthesized DNA, analyzed by Sanger sequencing and NGS.

DNA Library Construction Services
(Combinatorial-OGAB™ (Combi-OGAB™))

Using our proprietary Combinatorial-OGAB™(Combi-OGAB™) method, we design and construct large-scale, diverse DNA libraries to meet our clients’ needs. This enables our clients to search for the optimal DNA cluster for material production in a short period of time and limited resources.

Order Flow

Please contact us using the inquiry form link at the bottom of the page, selecting either “DNA Synthesis” or “DNA Library Construction”.

Select “DNA synthesis” or “DNA Library Construction” from the inquiry form and provide us with the details of your request.

We will send you an email on how to provide us with sequence information, along with an Excel file for requesting a quote. Please fill in the required information and send it back to us.

We will send you instructions on how to send and receive encrypted sequence information to the e-mail address that you have entered in the inquiry form.

After receiving the file, we carefully examine whether we can synthesize the sequence.

Our technical staff will determine whether or not the product can be synthesized, and our safety committee will determine whether or not the product can be handled. Please answer each of the following questions on the inquiry form to confirm the presence or absence of pathogenic sequences.

If we find that the sequence you have requested can be synthesized, we will send you a quotation. Once you have accepted the quotation and placed your order, we will begin the synthesis work.

We will send you a quotation. We will start the commissioned synthesis after the order has been placed.

CONTACT

If you have any questions about our services and technologies,
or if you would like to discuss collaboration, media coverage,
etc., please contact us using the form below.

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